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價格:電議
所在地:上海
型號:23966-2
更新時間:2021-08-11
瀏覽次數(shù):1562
公司地址:上海市徐匯區(qū)龍吳路2588弄54號1002室
楊超(先生) 經(jīng)理
Polyethylenimine,Linear,Mw 25,000
線性化聚乙烯亞胺(PEI 25,000)
基本描述:
線性化聚乙烯亞胺PEI 25,000,一種高電荷陽離子聚合物,非常容易結(jié)合于高電荷陰離子基質(zhì)。工業(yè)應(yīng)用上,線性化PEI可改善負(fù)電荷染料的物理外觀,以調(diào)整染料的化學(xué)特性和增強染料與固相基質(zhì)的黏附能力,常用作黏附增強劑,作用同多聚賴氨酸(poly-lysine);科研應(yīng)用上,線性化PEI能與DNA或其他負(fù)電荷生物大分子簡單結(jié)合,正是這一特性,使得成為一種非常行之有效的載體運輸介質(zhì)(Vector carrier),即轉(zhuǎn)染試劑。
化學(xué)性質(zhì):
CAS NO. |
9002-98-6, 26913-06-4 |
Formula |
(CH2CH2NH)n |
Molecular Weight |
25,000 |
Melting Point |
73-75o |
Solubility |
Soluble In: hot water, cold water at low pH, methanol, and ethanol. Insoluble In: benzene, ethyl ether and acetone |
Appearance |
White to yellow solid |
訂購信息:【原裝進口,現(xiàn)貨供應(yīng)】
品牌 |
貨號 |
名稱 |
規(guī)格 |
促銷價(元) |
Polysciences |
23966-2 |
線性聚乙烯亞胺(MW 25,000) |
2g |
咨詢 |
另,提供更高轉(zhuǎn)化效率的PEI Max 40,000,自由基形式分子量相當(dāng)于PEI 25,000,研究證實其體外質(zhì)粒DNA轉(zhuǎn)染效率提高達21倍。 |
||||
Polysciences |
24765-2 |
線性聚乙烯亞胺鹽酸鹽Max,(MW 40,000) (Equivalent to Mw 25,000 in Free ba |
2g |
咨詢 |
使用方法【來源于:CSH protocols,僅作交流學(xué)習(xí)】 :
1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.
2. Add 500 mg of PEI to the beaker with stirring.
3. Add co
4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.
Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.
5. Add co
Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.
6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.
7. Filter-sterilize the solution through a 0.22-μm membrane.
8. Store aliquots of the desired volume at -20°C.
應(yīng)用文獻【基因轉(zhuǎn)染用途】 :
Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.
Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pintel (2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors. Journal of Virological Methods. 138(1–2): 85–98. Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy(2013)Transient Mammalian Cell Transfection with Polyethylenimine (PEI) . Methods Enzymol. 529: 227–240.
——Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
上海懋康生物科技有限公司是一家涉足于生命科學(xué)和生物技術(shù)領(lǐng)域研究的試劑、儀器和實驗室消耗品與實驗服務(wù)工作,主要從事細(xì)胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。 本公司秉承“以人為本,以誠為信、合同守信”的經(jīng)營理念。堅持"品質(zhì)保障"的原則為廣大客戶提供優(yōu)質(zhì)產(chǎn)品。
線性化聚乙烯亞胺(MW 25,000)PEI 25000
線性化聚乙烯亞胺(MW 25,000)PEI 25000
線性化聚乙烯亞胺(MW 25,000)PEI 25000